5aMU3 – The Origins of Building Acoustics for Theatre and Music Performances

John Mourjopoulos – mourjop@upatras.gr
University of Patras
Audio & Acoustic Technology Group,
Electrical and Computer Engineering Dept.,
26500 Patras, Greece

Historical perspective
The ancient open amphitheatres and the roofed odeia of the Greek-Roman era present the earliest testament of public buildings designed for effective communication of theatrical and music performances over large audiences, often up to 15000 spectators [1-4]. Although mostly located around the Mediterranean, such antique theatres were built in every major city of the ancient world in Europe, Middle East, North Africa and beyond. Nearly 1000 such buildings have been identified, their evolution starting possibly from the Minoan and archaic times, around 12th century BC. However, the known amphitheatric form appears during the age that saw the flourishing of philosophy, mathematics and geometry, after the 6th century BC. These theatres were the birthplace of the classic ancient tragedy and comedy plays fostering theatrical and music activities for at least 700 years, until their demise during the early Christian era. After a gap of 1000 years, public theatres, opera houses and concert halls, often modelled on these antique buildings, re-emerged in Europe during the Renaissance era.

During the antiquity, open theatres were mainly used for staging drama theatrical performances so that their acoustics were tuned for speech intelligibility allowing very large audiences to hear clearly the actors and the singing chorus. During this era, smaller sized roofed versions of these theatres, the “odeia” (plural for “odeon”), were also constructed [4, 5], often at close vicinity to open theatres (Figure 1). The odeia had different acoustics qualities with strong reverberation and thus were not appropriate for speech and theatrical performances but instead were good for performing music functioning somehow similarly to modern-day concert halls.

Mourjopoulous1 - odeia
Figure 1: representation of buildings around ancient Athens Acropolis during the Roman era. Besides the ancient open amphitheatre of Dionysus, the roofed odeion of Pericles is shown, along with the later period odeion of Herodes (adopted from www.ancientathens3d.com [6]).

Open amphitheatre acoustics for theatrical plays
The open antique theatre signifies the initial meeting point between architecture, acoustics and the theatrical act. This simple structure consists of the large truncated-cone shaped stepped audience area, (the amphitheatrical “koilon” in Greek or “cavea” in Latin), the flat stage area for the chorus (the “orchestra”) and the stage building (the “skene”) with the raised stage (“proskenion”) for the actors (Figure 2).

Mourjopoulous2
Figure 2: structure of the Hellenistic period open theatre.

The acoustic quality of these ancient theatres amazes visitors and experts alike. Recently, the widespread use of acoustic simulation software and of sophisticated computer models has allowed a better understanding of the unique open amphitheatre acoustics, even when the theatres are known solely from archaeological records [1,3,7,9,11]. Modern portable equipment has allowed state-of-the-art measurements to be carried out in some well-preserved ancient theatres [8,10,13]. As a test case, the classical / Hellenistic theatre of Epidaurus in southern Greece is often studied which is famous for its near-perfect speech intelligibility [12,13]. Recent measurements with audience present (Figure 3) confirm that intelligibility is retained besides the increased audience sound absorption [13].

Mourjopoulous3
Figure 3: Acoustic measurements at the Epidaurus theatre during recent drama play (form Psarras et al.[13]).

It is now clear that the “good acoustics” of these amphitheatres and especially of Epidaurus, is due to a number of parameters: sufficient amplification of stage sound, uniform spatial acoustic coverage, low reverberation, enhancement of voice timbre, all contributing to perfect intelligibility even at seats 60 meters away, provided that environmental noise is low. These acoustically important functions are largely a result of the unique amphitheatrical shape: for any sound produced in the stage or the orchestra, the geometric shape and hard materials of the theatre’s surfaces generate sufficient reflected and scattered sound energy which comes first from the stage building (when this exists), then the orchestra floor and finally from the surfaces at the top and back of seat rows adjacent each listener position and which is uniformly spread to the audience area [11,13] (see Figure 4 and Figure 5).

Mourjopoulous4
Figure 4: Acoustic wave propagation 2D model for the Epidaurus theatre. The blue curves show the direct and reflected waves at successive time instances indicated by the red dotted lines. Along with the forward propagating wavefronts, backscattered and reflected waves from the seating rows are produced (from Lokki et al. [11]).

This reflected sound energy reinforces the sound produced in the stage and its main bulk arrives at the listener’s ears very shortly, typically within 40 milliseconds after the direct signal (see Figure 5). Within such short intervals, as far as the listeners’ brain is concerned, this is sound also coming from the direction of the source in the stage, due to a well-known perceptual property of human hearing, often referred to as “precedence or Haas effect” [11,13].

Mourjopoulous5
Figure 5: Acoustic response measurement for the Epidaurus theatre, assuming that the source emits a short pulse and the microphone is at a seat at 15 meters. Given that today the stage building does not exist, the first reflection arrives very shortly from the orchestra ground. Seven successive and periodic reflections can be seen from the top and the risers of adjacent seat rows. Their energy is reduced within approx. 40 milliseconds after the arrival of the direct sound (from Vassilantonopoulos et al. [12]).

The dimensions for seating width and riser height, as well as the koilon slope, can ensure minimal sound occlusion by lower tiers and audience and result to the fine tuning of in-phase combinations of the strong direct and reflected sounds [9,11]. As a result, frequencies useful for speech communication are amplified adding a characteristic coloration of voice sound and further assisting clear speech perception [11]. These specific amphitheatre design details have been found to affect the qualitative and quantitative aspects of amphitheatre acoustics and in this respect, each ancient theatre has unique acoustic character. Given that the amphitheatric seating concept evolved from earlier archaic rectangular or trapezoidal shaped seating arrangements with inferior acoustics (see Figure 6), such evolution hints at possible conscious acoustic design principles employed by the ancient architects. During the Roman period, stage building grew in size and the orchestra was truncated, showing adaptation to artistic, political and social trends with acoustic properties correlated to intended new uses favouring more the visual performance elements [4,15]. Unfortunately, only few fragments of such ancient acoustic design principles have been found and only via the writings of the Roman architect Marcus Vitruvius Pollio (70-15 BC), [14].

Mourjopoulous6
Figure 6: Evolution of the shape of open theatres. Roman period theatres had semi-circular orchestra and taller and more elaborate stage building.The red lines indicate the koilon / orchestra design principle as described by the ancient architect Vitruvius.

The acoustics of odeia for music performances
Although the form of ancient odeia broadly followed the amphitheatric seating and stage / orchestra design, they were covered by roofs usually made from timber. This covered amphitheatric form was also initially adopted by the early Renaissance theatres, nearly 1000 years after the demise of antique odeia [16] (Figure 7).

Mourjopoulous7
Figure 7: Different shapes of roofed odeia of antiquity and the Renaissance period (representations from www.ancientathens3d.com [6]).

Supporting a large roof structure without any inner pillars over the wide diameter dictated by the amphitheatric shape, presents even today a structural engineering feat and it is no wonder that odeia roofs are not preserved. Without their roofs, these odeia appear today to be similar to the open amphitheatres. However, computer simulations indicate that in period, unlike the open theatres, they had strong acoustic reverberation and their acoustics helped the loudness and timbre of musical instruments at the expense of speech intelligibility, so that these spaces were not appropriate and were not used for theatrical plays [4,5]. For the case of the Herodes odeion in Athens (Figure 8), computer simulations show that the semi-roofed version had up to 25% worst speech intelligibility compared to the current open state, but the strong acoustic reverberation which was similar to a modern concert hall of compatible inner volume of 10000 m3, made it suitable as a music performance space [5].

Mourjopoulous8
Figure 8: The Herodes odeion at its current state and via computer model of the current open and its antique semi-roofed version. (from Vassilantonopoulos et al. [5]). Very recent archaeological evidence indicates that the roof covered fully the building, as is also shown in Figure 10.

Thousand years ago, these antique theatres established acoustic functionality principles that even today prevail for the proper presentation of theatre and music performances to public audiences and thus signal the origins of the art and science in building acoustics.

A virtual acoustic tour of simulated amphitheatres and odeia is available at:
http://www.ancientacoustics2011.upatras.gr/Files/ANC_THE_FLASH/index.html
Please use headphones for more realistic 3D sound effect.

References
[1] F. Canac, “L’acoustique des théâtres antiques”, published by CNRS, Paris, (1967).
[2] R. Shankland, “Acoustics of Greek theatres”, Physics Today, (1973).
[3] K. Chourmouziadou, J. Kang, “Acoustic evolution of ancient Greek and Roman theatres”, Applied Acoustics vol.69 (2008).
[4] G. C. Izenur, “Roofed Theaters of Classical Antiquity”, Yale University Press, New Haven, Connecticut, (1992).
[5] S. Vassilantonopoulos, J. Mourjopoulos, “The Acoustics of Roofed Ancient Odea”, Acta Acoustica united with Acustica, vol.95, (2009).
[6] D. Tsalkanis, www.ancientathens3d.com, (accessed April 2015).
[7] S. L. Vassilantonopoulos, J. N. Mourjopoulos, “A study of ancient Greek and Roman theater acoustics”, Acta Acustica united with Acustica 89 (2002).
[8] A.C. Gade, C. Lynge, M. Lisa, J.H.Rindel, “Matching simulations with measured acoustic data from Roman theatres using the ODEON programme”, Proceedings of Forum Acusticum 2005, (2005).
[9] N. F. Declerq, C. S. Dekeyser, “Acoustic diffraction effects at the Hellenistic amphitheatre of Epidaurus: Seat rows responsible for the marvellous acoustics”, J. Acoust. Soc. Am. 121 (2007).
[10] A. Farnetani, N. Prodi, R. Pompoli, “On the acoustics of ancient Greek and Roman theatres”, J. Acoust. Soc. Am. 124 (2008).
[11] T. Lokki, A. Southern, S. Siltanen, L. Savioja, “Studies of Epidaurus with a hybrid room acoustics modelling method”, Acta Acustica united with Acustica, vol.99, 2013.
[12] S. Vassilantonopoulos, T. Zakynthinos, P. Hatziantoniou, N.-A. Tatlas, D. Skarlatos, J. Mourjopoulos, “Measurement and analysis of acoustics of Epidaurus theatre” (in Greek), Hellenic Institute of Acoustics Conference, (2004).
[13] S. Psarras, P. Hatziantoniou, M. Kountouras, N-A. Tatlas, J. Mourjopoulos, D. Skarlatos, “Measurement and Analysis of the Epidaurus Ancient Theatre Acoustics”, Acta Acustica united with Acustica, vol.99, (2013).
[14] Vitruvius, “The ten books on architecture” (translated by Morgan MH), London / Cambridge, MA: Harvard University Press, (1914).
[15] Beckers, Benoit, N.Borgia, “The acoustic model of the Greek theatre.” Protection of Historical Buildings, Prohitech09, (2009).
[16] M. Barron, “Auditorium acoustics and architectural design”, London: E& FN Spon (1993).

2pNSb – A smartphone noise meter app in every pocket?

Chucri A. Kardous – ckardous@cdc.gov
Peter B. Shaw – pbs3@cdc.gov
National Institute for Occupational Safety and Health
Centers for Disease Control and Prevention
1090 Tusculum Avenue
Cincinnati, Ohio 45226

Popular version of paper 2pNSb, “Use of smartphone sound measurement apps for occupational noise assessments”
Presented Tuesday May 19, 2015, 3:55 PM, Ballroom 1
169th ASA Meeting, Pittsburgh, PA
See also: Evaluation of smartphone sound measurement applications

Our world is getting louder. Excessive noise is a public health problem and can cause a range of health issues; noise exposure can induce hearing impairment, cardiovascular disease, hypertension, sleep disturbance, and a host of other psychological and social behavior problems. The World Health Organization (WHO) estimates that there are 360 million people with disabling hearing loss. Occupational hearing loss is the most common work-related illness in the United States; the National Institute for Occupational Safety and Health (NIOSH) estimates that approximately 22 million U.S. workers are exposed to hazardous noise.

Smartphones users are expected to hit the 2 billion mark in 2015. The ubiquity of smartphones and the sophistication of current sound measurement applications (apps) present a great opportunity to revolutionize the way we look at noise and its effects on our hearing and overall health. Through the use of crowdsourcing techniques, people around the world may be able to collect and share noise exposure data using their smartphones. Scientists and public health professionals could rely on such shared data to promote better hearing health and prevention efforts. In addition, the ability to acquire and display real-time noise exposure data raises people’s awareness about their work (and off-work) environment and allows them to make informed decisions about hazards to their hearing and overall well-being. For instance, the European Environment Agency (EEA) developed the Noise Watch app that allows citizens around the world to make noise measurements whether at their work or during their leisure activities, and upload that data to a database in real time and using the smartphone GPS capabilities to construct a map of the noisiest places and sources in their environment.

However, not all smartphone sound measurements apps are equal. Some are basic and not very accurate while some are much more sophisticated. NIOSH researchers conducted a study of 192 smartphone sound measurement apps to examine the accuracy and functionality of such apps. We conducted the study in our acoustics laboratory and compared the results to a professional sound level meter. Only 10 apps met our selection criteria, and of those only 4 met our accuracy requirements of being within ±2 decibels (dB) of type 1 professional sound level meter. Apps developed for the iOS platform were more advanced, functionality and performance wise, than Android apps. You can read more about our original study on our NIOSH Science Blog at: http://blogs.cdc.gov/niosh-science-blog/2014/04/09/sound-apps/ or download our JASA paper at: http://scitation.aip.org/content/asa/journal/jasa/135/4/10.1121/1.4865269.

Testing the SoundMeter app on the iPhone 5 and iPhone 4S
Figure 1. Testing the SoundMeter app on the iPhone 5 and iPhone 4S against a ½” Larson-Davis 2559 random incidence reference microphone
Today, we will present on our additional efforts to examine the accuracy of smartphone sound measurement apps using external microphones that can be calibrated. There are several external microphones available mostly for the consumer market, and although they vary greatly in price, they all possess similar acoustical specifications and have performed similarly in our laboratory tests. Preliminary results showed even greater agreement with professional sound measurement instruments (± 1 dB) over our testing range.

Calibrating the SPLnFFT app
Figure 2. Calibrating the SPLnFFT app with MicW i436 external microphone using the Larson-Davis CAL250 acoustic calibrator (114 dB SPL @ 250Hz)

Figure 3

Figure 3. Laboratory testing of 4 iOS devices using MicW i436 and comparing the measurements to a Larson-Davis type 831 sound level meter (pink noise at 75 dBA)

We will also discuss our plans to develop and distribute a free NIOSH Sound Level Meter app in an effort to facilitate future occupational research efforts and build an noise job exposure database.

Challenges remain with using smartphones to collect and document noise exposure data. Some of the main issues encountered in recent studies relate to privacy and collection of personal data, sustained motivation to participate in such studies, bad or corrupted data, and mechanisms for storing and accessing such data.

2aPP6 – Emergence of Spoken Language in Deaf Children Receiving a Cochlear Implant

Ann E. Geers
Popular version of 2aPP6. Language emergence in early-implanted children
Presented at the 169th Meeting of the Acoustical Society of America
May 2015

Before the advent of Cochlear Implants (CI), children who were born profoundly deaf acquired spoken language and literacy skills with great difficulty and over many years of intensive education. Even with the most powerful hearing aids and early intervention, children learned spoken language at about half the normal rate, and fell further behind in language and reading with increasing age. At that time, many deaf children learned to communicate through sign language, though more than 90% of them had parents with normal hearing who did not know how to sign when their deaf child was born.

Following FDA approval in the 1990s, many deaf children began receiving a CI (in one ear) at some point after their second birthday. Dramatic improvements were seen compared to hearing aid users in the ability to hear and produce clear speech, understand spoken language and acquire literacy skills. However many children with CIs still did not reach levels within the range of their age mates with normal hearing in these areas. Over the next 2 decades, with universal newborn hearing screening mandatory in most states, implantation occurred at younger ages (typically 12-18 months) and CI technology offered improved access to speech, especially soft sounds. As implant performance continued to improve for children receiving one CI, receiving a second CI to optimize hearing at both ears was considered.

This study followed 60 children implanted between 12 and 38 months of age when they were 3, 4 and 10 years old. All of them were in preschool programs focused on developing spoken language skills and had no disabilities other than hearing impairment. By age 10, 95% of them were enrolled in regular education settings with hearing age mates.

Three groups, roughly equal in size, were identified from standardized language tests administered at 4 and 10 years of age. 1) Normal Language Emergence – these children exhibited spoken language skills within the normal range by age 4 and continued along this normal course into their elementary school years. They developed above-average reading comprehension. 2) Late Language Emergence – these children were language-delayed in preschool, but caught up by the time they were 10. They developed average reading comprehension for their age. 3) Persistent Language Delay- these children were also language-delayed in preschool, but they did not catch up with hearing age-mates by age 10. They were below-average readers.

Achieving age-appropriate language and reading skills by mid-elementary grades is a remarkable accomplishment for children with profound hearing loss and the fact that two-thirds of the sample reached or exceeded this level attests to the efficacy of early cochlear implantation. In fact, children with normal language emergence were most likely to have received a CI very young – between 12 and 18 months of age. However, age at first CI did not differentiate children with late language emergence from those with persistent delay. In fact, these groups did not differ in nonverbal intelligence, mother’s education, bilateral implantation, age at first intervention or age enrolled in regular education classrooms. As a result, predicting during preschool whether or not a child will catch up with hearing children in the same grade is difficult. We looked for factors distinguishing language-delayed preschoolers who would reach age-appropriate language levels by mid-elementary grades from those who would remain delayed. Early prediction is important for intensifying and individualizing early intervention for children at risk for long-term delay.

Results from a battery of tests and questionnaires revealed a constellation of factors distinguishing children with persistent from those with resolving language delay. Most of these factors were associated with the quality of the audio input provided by the device. For example, odds were 3-4 times greater that children who caught up used more recent CI technology than those who remained delayed. Children who caught up in language had a particular advantage in their ability to detect and understand speech presented at soft levels. This is understandable, because incidental or casual language acquisition depends on the ability to overhear soft speech in addition to speech at normal-conversation levels. In addition, a smaller repertoire of speech sounds, lower vocabulary and poorer grammar skills were evident in the conversational language of persistently delayed children as early as 3 years of age with smaller language gains between 3 and 4 years, foreshadowing slower long-term speech and language development. A somewhat surprising finding was that a much larger percentage (47%) of persistently delayed children had left-ear CIs as compared with those who caught up (14%).

These results have important implications for surgeons, speech-language pathologists, educators and audiologists serving young children with cochlear implants. For the surgeon, right-ear placement of the first CI should be preferred over the left unless cochlear anatomy precludes placement at the right ear. This, along with implantation by 18 months, may help to maximize chances of age-appropriate spoken language development. For the speech language pathologist, the extent of immature speech production and language use during preschool years may foreshadow later language difficulties. For the audiologist, encouraging upgraded speech processor technology and working to ensure the audibility of soft speech when programming the device may positively influence future language development. For the educator, recognition of risk factors for persistent language delay may signal increased intensity of language intervention. Addressing these issues should increase the likelihood that children with CIs will exhibit spoken communication and academic skills in line with expectations for their grade placement.

2aNSa1 – Soundscape will tune an acoustic environment through peoples’ mind

Brigitte Schulte-Fortkamp – b.schulte-fortkamp@tu-berlin.de
Technical University Berlin
Institute of Fluid Mechanics and Engineering Acoustics
-Psychoacoustics and Noise effects –
Einsteinufer 25
10587 Berlin -Germany

Popular version of paper 2aNSa1, “Soundscape as a resource to balance the quality of an acoustic environment”
Tuesday morning, May 19, 2015, 8:35 AM, Commonwealth 1
169th ASA Meeting, Pittsburgh Pennsylvania

Preface
Soundscape studies investigate and find increasingly better ways to measure and hone the acoustic environment. Soundscape offers the opportunity for multidisciplinary working, bringing together science, medicine, social studies and the arts – combined, crucially, with analysis, advice and feedback from the ‘users of the space’ as the primary ‘experts’ of any environment – to find creative and responsive solutions for protection of living places and to enhance the quality of life.

The Soundscape concept was introduced as a scope to rethink the evaluation of “noise” and its effects. The challenge was to consider the limits of acoustic measurements and to account for its cultural dimension.

The recent international standard ISO 12913-1 Acoustics — Soundscape —Part 1: Definition and conceptual framework Acoustique – Paysage sonore -Partie 1: Définition et cadre conceptual clarifies soundscape as an “acoustic environment as perceived or experienced and/or understood by a person or people, in context”

Soundscape
Figure 1 — Elements in the perceptual construct of soundscape

Soundscape suggests exploring noise in its complexity and its ambivalence and its approach towards sound to consider the conditions and purposes of its production, perception, and evaluation, to understand evaluation of noise/ sound as a holistic approach.

To discuss the contribution of Soundscape research into the area of Community noise research means to focus on the meaning of sounds and its implicit assessments to contribute to the understanding that the evaluation through perceptual effects is a key issue.

Using the resources – an example-
Soundscape Approach Public Space Perception and Enhancement Drawing on Experience in Berlin
Slide1 - Soundscape
Figure 2 – Soundscape Nauener Platz

The concept of development of the open pace relies on the understanding that people living in the chosen are the “real” experts concerning the evaluation of this place according to their expectations and experiences in the respective area. The intention of scientific research here is to learn about the meaning of the noise with respect to people’s living situation and to implement the adequate procedure to open the “black box” of people’s mind.

Therefore, the aim was to get residents involved through workshops to get access to the different social groups.
Slide4
Figure 3 – Participation and Collaboration
Slide3
Figure 4 – The concept of evaluation

Interdisciplinarity is considered as a must in the soundscape approach. In this case it was concerned with the collaboration of architects, acoustics engineers, environmental health specialists, psychologists, social scientists, and urban developers. The tasks are related to the local individual needs and are open to noise sensitive and other vulnerable groups. It is also concerned with cultural aspects and the relevance of natural soundscapes – sometimes referred to as quiet areas – which is obviously related to the highest level of needs.
Slide6
Figure 5 – Soundscape – an interactive approach using the resources

Improving local soundscape quality?
Obviously, these new approaches and methods make it possible to learn about the process of perception and evaluation sufficiently as they take into account the context, ambiance, the usual interaction between noise and listener and the multidimensionality of noise perception.

By contrast, conventional methods often reduce the complexity of reality on controllable variables, which supposedly represent the scrutinized object. Furthermore, traditional tests neglect frequently the context-dependency of human perception; they only provide artificial realities and diminish the complexity of perception on merely predetermined values, which do not completely correspond with perceptual authenticity. However, perception and evaluations entirely depend on the respective influences of the acoustic and non-acoustic modifiers.

Following the comments and group discussion and also the results from the narrative interviews it could be defined why people prefer some places over the public place and why not. It also became clear how people experience the noise in the distance from the road and also with respect to social life and social control. One of the most important findings here is how people react to low frequency noise at the public place and how experiences and expectations work together. It becomes obvious that the most wanted sound in this area is based on wishes to escape the road traffic noise through natural sounds.
Slide5
Figure 6 – Selected sounds for audio islands

Reshaping the place based on people’s expertise
Relying on the combined evaluation procedures the place was reshaped installing a gabion wall along one of the main roads and further more audio islands like have been built that integrated the sounds people would like to enjoy when using the place. While the gabion wall protects against noise around the playground, the new installed audio islands provide nature sounds as selected by the people involved in the Soundscape approach.
Slide7
Figure 7 – Installation of the sounds

Conclusions
Slide8
Figure 8 – The new place

The process of tuning of urban areas with respect to the expertise of people’s mind and quality of life is related to the strategy of triangulation and provides the theoretical frame with regard to the solution of e.g. the change in an area. In other words: Approaching the field in this holistic manner is generally needed.

An effective and sustainable reduction of the number of highly annoyed people caused by noise is only possible with further scientific endeavors in the area of methods development and research of noise effects. Noise maps providing further information can help to obtain a deeper understanding of noise reactions and can help to reliably identify perception-related hot spots. Psychoacoustic maps are particularly interesting in areas where the noise levels are marginal below the noise level limits and offer an additional interpretation help with respect to the identification of required noise abatement measures.

But, the expertise of people involved will provide meaningful information. Soundwalks as an eligibly instrument for exploring urban areas by minds of the “local experts” as measuring device open a field of data for triangulation. These techniques in combination allow giving meaning to the numbers and values of recordings and their analysis to understand the significance of sound and noise as well as the perception of Soundscapes by its resources.
tags: soundscape, acoustics, people, health

REFERENCES
J. Kang, B. Schulte-Fortkamp (editors) Soundscape and the Built Environment CRC Press | Taylor & Francis Group, in print
B. Schulte-Fortkamp, J. Kang (editors) Special Issue on Soundscape, JASA 2012
R. M. Schafer, “The Soundscape. Our sonic environment and the tuning of the world.” Rochester, Vermont: Destiny Books, (1977).
B. Hollstein, “Qualitative approaches to social reality: the search for meaning” in: John Scott & Peter J. Carrington (Eds.): Sage handbook of social network analysis. London/Newe Dehli: Sage. (2012)
R. M. Schafer, “The Book of Noise” (Price Milburn Co., Lee, Wellington, NZ, (1973).
B. Truax, (ed.) „Handbook for Acoustic Ecology” (A.R.C. Publication, Vancouver, (1978).
K. Hiramatsu, “Soundscape: The Concept and Its Significance in Acoustics,” Proc. ICA, Kyoto, 2004.
A. Fiebig, B. Schulte-Fortkamp, K. Genuit, „New options for the determination of environmental noise quality”, 35th International Congress and Exposition on Noise Control Engineering INTER-NOISE 2006, 04.-06.December 2006, Honolulu, HI.
P. Lercher, B. Schulte-Fortkamp, “Soundscape and community noise annoyance in the context of environmental impact assessments,” Proc. INTER-NOISE 2003, 2815-2824, (2003).
B. Schulte-Fortkamp, D. Dubois: (editors) Acta Acustica united with Acustica, Special Issue, Recent advances in Soundscape research, Vol 92 (6), (2006).
R. Klaboe, et. al. „Änderungen in der Klang- und Stadtlandschaft nach Änderung von Straßenverkehrsstraßen im Stadtteil Oslo-Ost“, Fortschritte der Akustik, Oldenburg, (2000).

3aBA5 – Fabricating Blood Vessels with Ultrasound

Diane Dalecki, Ph.D.
Eric S. Comeau, M.S.
Denise C. Hocking, Ph.D.
Rochester Center for Biomedical Ultrasound
University of Rochester
Rochester, NY 14627

Popular version of paper 3aBA5, “Applications of acoustic radiation force for microvascular tissue engineering”
Presented Wednesday morning May 20, 9:25 AM, in room Kings 2
169th ASA Meeting, Pittsburgh

Tissue engineering is the field of science dedicated to fabricating artificial tissues and organs that can be made available for patients in need of organ transplantation or tissue reconstructive surgery. Tissue engineers have successfully fabricated relatively thin tissues, such as skin substitutes, that can receive nutrients and oxygen by simple diffusion. However, recreating larger and/or more complex tissues and organs will require developing methods to fabricate functional microvascular networks to bring nutrients to all areas of the tissue for survival.

In the laboratories of Diane Dalecki, Ph.D. and Denise C. Hocking, Ph.D., research is underway to develop new ultrasound technologies to control and enhance the fabrication of artificial tissues1. Ultrasound fields are sound fields at frequencies higher than humans can hear (i.e., > 20 kHz). Dalecki and Hocking have developed a technology that uses a particular type of ultrasound field, called an ultrasound standing wave field, as a tool to non-invasively engineer complex spatial patterns of cells2 and fabricate microvessel networks3,4 within artificial tissue constructs.

When a solution of collagen and cells is exposed to an ultrasound standing wave field, the forces associated with the field lead to the alignment of the cells into planar bands (Figure 1). The distance between the bands of cells is controlled by the ultrasound frequency, and the density of cells within each band is controlled by the intensity of the sound field. The collagen polymerizes into a solid gel during the ultrasound exposure, thereby maintaining the spatial organization of the cells after the ultrasound is turned off. More complex patterning can be achieved by use of more than one ultrasound transducer.

Dalecki-1-ASA

Figure 1. Acoustic-patterning of microparticles (dark bands) using an ultrasound standing wave field. Distance between planar bands is 750 µm. Scale bar = 100 μm

An exciting application of this technology involves the fabrication of microvascular networks within artificial tissue constructs. Specifically, acoustic-patterning of endothelial cells into planar bands within collagen hydrogels leads to the rapid development of microvessel networks throughout the entire volume of the hydrogel. Interestingly, the structure of the resultant microvessel network can be controlled by choice of the ultrasound exposure parameters. As shown in Figure 2, ultrasound standing wave fields can be employed to fabricate microvessel networks with different physiologically relevant morphologies, including capillary-like networks (left panel), aligned non-branching vessels (center panel) or aligned vessels with hierarchically branching microvessels. Ultrasound fields provide an ideal technology for microvascular engineering; the technology is rapid, noninvasive, can be broadly applied to many types of cells and hydrogels, and can be adapted to commercial fabrication processes.

Dalecki-2-ASA - Ultrasound-fabricated microvessel

Figure 2. Ultrasound-fabricated microvessel networks within collagen hydrogels. The ultrasound pressure amplitude used for initial patterning determines the final microvessel morphology, which can resemble torturous capillary-like networks (left panel), aligned non-branching vessels (center panel) or aligned vessels with hierarchically branching microvessels. Scale bars = 100 μm.

To learn more about this research, please view this informative video (https://www.youtube.com/watch?v=ZL-cx21SGn4).

References:

[1] Dalecki D, Hocking DC. Ultrasound technologies for biomaterials fabrication and imaging. Annals of Biomedical Engineering 43:747-761; 2015.

[2] Garvin KA, Hocking DC, Dalecki D. Controlling the spatial organization of cells and extracellular matrix proteins in engineered tissues using ultrasound standing wave fields. Ultrasound Med. Biol. 36:1919-1932; 2010.

[3] Garvin KA, Dalecki D, Hocking DC. Vascularization of three-dimensional collagen hydrogels using ultrasound standing wave fields. Ultrasound Med. Biol. 37:1853-1864; 2011.

[4] Garvin KA, Dalecki D, Youssefhussien M, Helguera M, Hocking DC. Spatial patterning of endothelial cells and vascular network formation using ultrasound standing wave fields. J. Acoust. Soc. Am. 134:1483-1490; 2013.